Volume 18, Issue 72 (1-2010)                   jour guilan uni med sci 2010, 18(72): 39-46 | Back to browse issues page

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Kazemi Arababadi M, Pourfathollah A, Hassanshahi G, Pooladvand V, Yaghini N, Shamsyzadeh A. Evaluation the Relationship between Alleles of +1188 in Region of IL-12 with Serum Level of Cytokine in Patients with Occult HBV Infection. jour guilan uni med sci. 2010; 18 (72) :39-46
URL: http://journal.gums.ac.ir/article-1-224-en.html
University of Medical Sciences
Abstract:   (4805 Views)
Abstract Introduction: Occult hepatitis B infection (OBI) is a form of hepatitis, which in despite of absence of detectable HBsAg, HBV-DNA is present in peripheral blood of patients. Objective: Evaluation the relationship between alleles of +1188 in region of IL-12 with serum level of cytokine in patients with occult HBV infection. Materials and Methods: In this study, the plasma samples of 3700 blood donors were tested for HBsAg and anti-HBc by ELISA. The HBsAg negative ve and anti-HBc positive samples were selected and screened for HBV-DNA by PCR. HBV-DNA positive samples assigned as OBI cases and PCR-SSP and ELISA were performed to examine the polymorphisms in region of (+1188 and serum level of IL-12) respectively. Results: The results showed that there is a significant difference in CC allele of +1188 region of IL-12 in two groups and no difference in the other evaluated genes. There is not any significant difference in serum level of IL-12 between OBI patients and controls. Our results also showed that there isn’t any significant statistically relation between alleles of +1188 region of IL-12 with serum level of cytokine. Conclusion: According to the results of this study it could be concluded that OBI patients unable to produce enough quantity of IL-12 and it may be related to different IL-12 gene. CC allele was associated with OBI, hence, it seems that +1188 region of IL-12 gene has an important role in expression of IL-12 gene. Evaluation of relation between polymorphisms in +1188 region of IL-12 gene and its expression. In vitro and under mitogene affect can useful because no association was seen between serum level of IL-12 and alleles of this region.
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Type of Study: Research | Subject: Special
Received: 2013/11/18 | Accepted: 2013/11/18 | Published: 2013/11/18

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