Volume 18, Issue 71 (10-2009)                   JGUMS 2009, 18(71): 1-6 | Back to browse issues page

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Ali Aghaei Shafi Abadi A, Seyyed Jaafari S. Differentiation of Human Placenta-derived Chorionic Stem Cells into Osteoblasts. JGUMS 2009; 18 (71) :1-6
URL: http://journal.gums.ac.ir/article-1-232-en.html
1- Kerman University of Medical Sciences , aghaei60@gmail.com
2- Kerman University of Medical Sciences
Abstract:   (7456 Views)
Abstract Introduction: Mesenchymal Stem Cells (MSCs) from various sources are capable of differentiating into different cell lineages under proper culture condition. Recently, human placenta appeared on the stage in the search for MSCs. Human derived chorionic villi stem cells, are cells with high proliferation and multipotential and will be differentiate other cells. Objective: Differentiation of Human Placenta-derived Chorionic Stem Cells into Osteoblasts. Materials and Methods: Chorionic derived Villi Stem Cells isolated from the human term placenta. Cells collected and cultured at DMEM/F12 medium. Osteogenic differentiation was induced with DMEM/F12 media(10%FBS) with ./1μM dexamethasone and 50μM Ascorbic Acid 3-Phosphate. Mineralized matrix was evaluated by von kossa staining. Results: The chorionic stem cells when plated, exhibited a population of fibroblast –like cells morphologically. This cells in osteogenic media containing Dexamethasone and Ascorbat Phosphate exhibited morphologic changes in cell structure after 7 days in culture. Cells changed from an elongated fibroblastic appearance to a rounded more cuboidal shape.The cells cultured with osteogenic factors were stained positively for mineralized nodular structures, as confirmed by von kossa staining. Secretion of calcified extracellular matrix was observed as black nodules. Secretion of cacified extracellular matrix was not observed in control group containing only chorionic stem cells without osteogenic media. Conclusion: we have reported osteogenic differentiation with using placenta derived chorionic stem cells. The results of these in vitro studies suggested that placenta derived chorionic stem cells one of the possible allogeneic sources for tissue engineering in the bone diseases
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Review Paper: Research | Subject: Special
Received: 2013/11/19 | Accepted: 2013/11/19 | Published: 2013/11/19

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