[Home ]     [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 26, Number 101 (journal of Guilan University of Medical Sciences 2017) ::
Back to browse issues page 3 2017, 26(101): 66-73
Modulation of Diazinon-Induced Oxidative Stress by Vitamins E and C in rat Brain
k Tahmasebi, Dr M Jafari *, M Salehi
Professor Baqiyatallah (a.s) University of Medical Sciences
Abstract:   (253 Views)

Introduction: Dazinon (DZN) as organophosphate insecticide induces oxidative stress. Vitamins E and C are antioxidants protecting cells from oxidative stress.

Objective: To determine the modulation of diazinon-induced oxidative stress by vitamins E and C in rat brain.

Materials and Methods: In present experimental study, male Wistar rats were randomly divided into six groups including: control (corn oil as DZN solvent), DZN (100 mg/kg), vitamin E (150 mg/kg), vitamin C (200 mg/kg), vitamin E+DZN and vitamin C+DZN groups, all of which were given intraperitoneally. 24 hours after injection, animals were anesthetized by ether, and brain tissue was quickly removed. After tissues hemogenation, superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST) and lactate dehydrogenase (LDH) activities, as well as glutathione (GSH) and malondialdehyde (MDA) levels were determined by biochemical methods.

Results: DZN increased SOD (p<0.001) and GST (p<0.01) activities and MDA level (p<0.01), while it decreased CAT and LDH activities and GSH content (p<0.01) in brain compared with the control group. Administration of vitamin E or vitamin C inhibited the changed in these parameters.

Discussion: Administration of vitamin E or vitamin C as antioxidant decreases DZN toxicity by scavenging free radicals but it does not protect completely.

Conflict of interest: none declared.

Keywords: Brain, Diazinon, Oxidative Stress, Rats, Vitamin C, Vitamin E, Brain, Rat
Full-Text [PDF 193 kb]   (138 Downloads)    
Type of Study: Research | Subject: Special
Received: 2017/04/30 | Accepted: 2017/04/30 | Published: 2017/04/30
Send email to the article author

Add your comments about this article
Your username or email:

Write the security code in the box >



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Tahmasebi K, Jafari M, Salehi M. Modulation of Diazinon-Induced Oxidative Stress by Vitamins E and C in rat Brain. 3. 2017; 26 (101) :66-73
URL: http://journal.gums.ac.ir/article-1-1403-en.html
Back to browse issues page Volume 26, Number 101 (journal of Guilan University of Medical Sciences 2017)
مجله دانشگاه علوم پزشکی گیلان Journal of Guilan University of Medical Sciences
Persian site map - English site map - Created in 0.146 seconds with 814 queries by yektaweb 3375