Volume 27, Issue 107 (11-2018)                   jour guilan uni med sci 2018, 27(107): 10-18 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ghafouri S, Nazemi A, Mokhtari G R. Detection of FGFR3 Gene S249C Mutation in Urinary Epithelial Cell by Tetra-Arms-Pcr Method in Patients With Bladder Tumors. jour guilan uni med sci. 2018; 27 (107) :10-18
URL: http://journal.gums.ac.ir/article-1-1710-en.html
Abstract:   (129 Views)
Abstract
Introduction: Genetic variation of FGFR3 gene is one of the factors affecting the bladder tumor. FGFR3 is a tyrosine kinase receptor, involved in controlling the cellular and angiogenesis cycle. This protein affects a variety of diseases and cancers and cartilage growth abnormalities. Regarding the high activity of fgfr3 mutations in more than 50% of primary tumors of bladder urethral cells, it was suggested in this stage that the gene is involved in tumor-forming epithelial cells.
Objective: the aim of this study was to detect S249C mutation in FGFR3 gene through tetra primers arms PCR and to determine the association between the presence of this mutation and the early stages of bladder cancer in the north of the country.
Materials and Methods: in this study, after separation of epithelial cell in the urine of 100 patients with bladder tumor, their DNA was extracted using a commercial kit. After bioinformatics studies, primers with appropriate pair of mutants were designed and synthesized for the S249C mutation. After optimization of the reaction, screening was performed on the population and the data were analyzed using SPSS software.
Results: Our results showed that primers designed to differentiate well between normal alleles and mutants in the epithelial cell of 28 patients with bladder tumor ,S249C mutations were identified p<0.0001. Statistical analysis of the data indicates the significance of this mutation.
Conclusion: The mutation of S249C FGFR3 gene was identified in 28% of the population studied and is considered a potential risk, although more and more studies are needed to confirm the results.
Conflict of interest: non declared
Full-Text [PDF 354 kb]   (58 Downloads)    
Type of Study: Research | Subject: Special
Received: 2018/10/27 | Accepted: 2018/10/27 | Published: 2018/10/27

Add your comments about this article : Your username or Email:
CAPTCHA code

© 2018 All Rights Reserved | Journal of Guilan University of Medical Sciences

Designed & Developed by : Yektaweb