Volume 16, Issue 61 (4-2007)                   jour guilan uni med sci 2007, 16(61): 1-8 | Back to browse issues page

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Mehrafza M, Heidarzadeh A, Jafari M, Oudi M, Aram R, Tavakol nia R et al . Survey of the Effect of Cervix Microbial Flora on Outcome of Intracytoplasmic Sperm Injection (ICSI). jour guilan uni med sci. 2007; 16 (61) :1-8
URL: http://journal.gums.ac.ir/article-1-391-en.html
Abstract:   (6704 Views)
Abstract Introduction: The cervix was colonized by potentially pathogenic microorganism that could be introduced into the uterine cavity or contaminate embryos during(Embryo Transfer). The consequences of this septic milieu on the outcome of embryo implantation is unclear. Objective: Survey the effect of microbial flora of the cervix on ICSI outcome at the time of embryo transfer in infertile women who referred to Mehr infertility institute. Materials and Methods: In this cross sectional descriptive study endo-cervical samples of 260 women were evaluated who under went intra-cytoplasmic sperm injection. After transferring the embryo were cut, about 3cm of the end of catheters and put in selective culture media. After incubating in special conditions by using current bacteriological methods bacterias were isolated & characterized. Pregnancy rate was confirmed by measurement of β-hCG in serum after 14 days. Some confounding variables (sperm quality, age, duration and cause of infertility, embryo quality) were controlled. Data was collected, analysis was carried out with T-test, chi squares test and multiple logistic regressions by using statistical soft ware SPSS.10. (P<0.05 was considered significant). Results: The results showed that overall PR by ICSI procedure was 40% (104 out of 260 cycles).The mean age of women was 31.6±6.2 years. The most common cause of infertility was related to male factor (46.2%). The mean infertility duration was 7.7±5.5 years. The quality of the most embryos were A (85%). 246(94.6%) of groups were negative culture and the others 14(5.4%) were positive culture. The most of research unit's (94.6%) culture were negative in 4.9% of pregnant women and in 5.8 of non pregnant women, results of culture test were positive. Multiple logistic regressions showed no significant effect of female's age, duration of infertility, cause of infertility, sperm parameters(count-morphology- motility),number of oocyte, number of MII, number of cleaved, number of ET, embryo quality & microbial culture in the outcome of ICSI but there was significant relation between female age & pregnancy rate. Conclusion: This study revealed that microbial flora of the cervix didn't influence in poor ICSI-ET outcome.
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Type of Study: Research | Subject: Special
Received: 2014/01/1 | Accepted: 2014/01/1 | Published: 2014/01/1

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