Volume 13, Issue 51 (10-2004)                   jour guilan uni med sci 2004, 13(51): 32-37 | Back to browse issues page

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Tokmehdashi H, Osati Ashtiani F, Purfathullah A. Comparison on Effect of Zinc on Viability and Morphology of Raji and Molt-4 Cell-Line. jour guilan uni med sci. 2004; 13 (51) :32-37
URL: http://journal.gums.ac.ir/article-1-652-en.html
Abstract:   (5004 Views)
Abstract Introduction: Zinc has important effects on structural and functional activities of many proteins and enzymes, especially in regulation of immune system. Objective: This study was carried out to examine the in vitro effects of different concentrations of Zinc on viability and morphology of T-Lymphoid (Molt-4) and B-Lymphoid (Raji) cell line. Materials and Methods: In this study, the cell line was exposed to different concentrations of Zinc (100μM to 500µM) followed by incubation (37ºC، 5%Co2) at various time points (12 to 72 hrs). The cells were then evaluated with trypan blue exclusion dye ، and Wright-Gimsa staining. Results: The results of this study showed almost different responses to different amounts of Zinc by the T and B cell line. Concentrations less than 100µM of Zinc at different incubation time points had little to no effects on cell line when compared to the controls. Higher concentrations of Zinc (>100µM) diminished cell viability to 70% at 12 hrs and less than 50% at 24 to 72 hrs of incubation times. Conclusion: We concluded that Zinc shows cytotoxic effects (Raji & Molt-4) depending on the concentrations used. At higher concentrations, it exerts cytotoxic effects.
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Type of Study: Research | Subject: General
Received: 2014/07/8 | Accepted: 2014/07/8 | Published: 2014/07/8

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