TY - JOUR T1 - Survey Prevalence of Group B Streptococci in Genital Tract Women in 28-37 Weeks Pregnancy TT - بررسی شیوع استرپتوکوک گروه B درمجاری تناسلی زنان باردار37-28 هفته JF - gums-med JO - gums-med VL - 15 IS - 59 UR - http://journal.gums.ac.ir/article-1-454-en.html Y1 - 2006 SP - 91 EP - 96 KW - Infection KW - Infant Mortality KW - Pregnancy KW - Streptococcus KW - Group B KW - Urinary Tract N2 - Abstract In‌troduction: Group B streptococci (GBS) have a tendency to colonize female genital tract and is a causing factor of premature delivery. In addition, they can also induce serious life – threa‌tening in‌fections such as meningitis and septe‌cemia in the newborn. GBS in‌fection are generally higher in pre-mature infants in relation to full-term born neorates. Objective: The purpose of this research was to determine the prevalence of Group B streptococci in genital tract 28-37 weeks of pregnant women. Materials and Methods: In this study specimens were obtained from 100 pregnant women (28 – 37 pregnancy weeks) who had not taken any antibiotics with‌in one-month period prior to sample collection and referred to prenatal ward and special clinic of Alzahra Hospital during summer 2005 in Rasht. Three vaginal swabs were taken from each woman. The first swab was used for direct lamb from vaginal secretions.The second swab was in‌oculated in 3ml of Todd – Hewitt broth (without antibiotic) and the third swab was inoculated into 3ml of selective Todd – Hewitt broth, supplemented gentamicin and Nalidixic acid. After 24h of in‌cubation at 37°C in 5% CO2 results of cultures were compared. For identification of isolated strains, the following tests were done, He‌molytic reaction, susceptibility to baci‌tracin, optochin and SXT discs, CAMP test, bile esculin, Hydrolysis of hippurate. Results: Based on various bio‌chemical and microbiologi‌cal tests, 15 GBS strains were isolated from the vaginal secretions of 100 pregnant women (28 – 37 pregnancy weeks). Fifteen strains of Streptococcus Viridans, One case of group A streptococcus Pnenmonea , 5 isolated belonging to the group C,G,F Streptococci, and 30 group D streptococci strains were isolated. Beside streptococci, other micro‌organisms were also isolated based on Gram staining and growth charac‌‌teristics on blood agar , and eosin methylene blue agar plates. One hundred Gram positive bacilli, 45 Gram negative bacilli, 60 staphylococci spp., and 40 yeast isolates were also detected. Conclusion: In attention to %15 of 28-37 weeks pregnancy women who were carriers, it is possible that it can cause premature delivery and also infections neonates. M3 ER -