AU - Rafiei, A.R AU - Karimi nia, A TI - Evaluation of Cellular Immunity of Patients with Brucellosis by Measuring Interferon Gamma (IFN-) and Interleukin-13 (IL-13) By Flow Cytometry and ELISA PT - JOURNAL ARTICLE TA - gums-med JN - gums-med VO - 14 VI - 55 IP - 55 4099 - http://journal.gums.ac.ir/article-1-517-en.html 4100 - http://journal.gums.ac.ir/article-1-517-en.pdf SO - gums-med 55 AB  - Abstract Introduction: The analysis of cytokine production is a valuable component of studies of immune response to stimulation such as pathogens, vaccines, and other immunological challenges. The animal findings Show that brucella infection induces cell-mediated responses. Some cytokines have an Important role in resistance to brucella infection. Objective: This Study is don’t to Poesent a orecise an useful unseparated whole blood of healthy normal and patients with acute and chronic brucellosis as the source of cells evaluation of interferon gamma (IFN-γ) and interleukin-13 expression. Materials and Methods: Diluted whole blood samples of 27 patients with acute (14) and chronic brucellosis (n13), and sex and age-matched healthy volunteers (n=22) With Meanage of 35-33+ 21 were cultured in the presence of either mitogen heat inactivated bacteria or medium alone. Intracellular IL-13 and IFN-γ were measured by specific sandwich ELISA and flow Cytometry was detected by the number of cytokine-producing CD3+ cells. Results: Findings indicated that extrandintracellular specific IFN-Y in creased Cansiderably (P CP - IRAN IN - LG - eng PB - gums-med PG - 87 PT - Research YR - 2005