TY - JOUR T1 - Evaluation of Cellular Immunity of Patients with Brucellosis by Measuring Interferon Gamma (IFN-) and Interleukin-13 (IL-13) By Flow Cytometry and ELISA TT - ارزیابی ایمنی سلولی بیماران مبتلا به بروسلوز با اندازه گیری گاما اینترفرون (IFN-) و اینترلوکین 13 (IL-13) با روش فلوسیتومتری و الایزا JF - gums-med JO - gums-med VL - 14 IS - 55 UR - http://journal.gums.ac.ir/article-1-517-en.html Y1 - 2005 SP - 87 EP - 96 KW - Brucellosis KW - Flow Cytometry KW - Interforous KW - Interleukins N2 - Abstract Introduction: The analysis of cytokine production is a valuable component of studies of immune response to stimulation such as pathogens, vaccines, and other immunological challenges. The animal findings Show that brucella infection induces cell-mediated responses. Some cytokines have an Important role in resistance to brucella infection. Objective: This Study is don’t to Poesent a orecise an useful unseparated whole blood of healthy normal and patients with acute and chronic brucellosis as the source of cells evaluation of interferon gamma (IFN-γ) and interleukin-13 expression. Materials and Methods: Diluted whole blood samples of 27 patients with acute (14) and chronic brucellosis (n13), and sex and age-matched healthy volunteers (n=22) With Meanage of 35-33+ 21 were cultured in the presence of either mitogen heat inactivated bacteria or medium alone. Intracellular IL-13 and IFN-γ were measured by specific sandwich ELISA and flow Cytometry was detected by the number of cytokine-producing CD3+ cells. Results: Findings indicated that extrandintracellular specific IFN-Y in creased Cansiderably (P M3 ER -