TY - JOUR JF - gums-med JO - JGUMS VL - 13 IS - 51 PY - 2004 Y1 - 2004/10/01 TI - Comparison on Effect of Zinc on Viability and Morphology of Raji and Molt-4 Cell-Line TT - مقایسه اثر روی (Zn )بر زنده ماندن و شکل ظاهری سلول های رده لنفوئیدی Raji و Molt-4 N2 - Abstract Introduction: Zinc has important effects on structural and functional activities of many proteins and enzymes, especially in regulation of immune system. Objective: This study was carried out to examine the in vitro effects of different concentrations of Zinc on viability and morphology of T-Lymphoid (Molt-4) and B-Lymphoid (Raji) cell line. Materials and Methods: In this study, the cell line was exposed to different concentrations of Zinc (100μM to 500µM) followed by incubation (37ºC، 5%Co2) at various time points (12 to 72 hrs). The cells were then evaluated with trypan blue exclusion dye ، and Wright-Gimsa staining. Results: The results of this study showed almost different responses to different amounts of Zinc by the T and B cell line. Concentrations less than 100µM of Zinc at different incubation time points had little to no effects on cell line when compared to the controls. Higher concentrations of Zinc (>100µM) diminished cell viability to 70% at 12 hrs and less than 50% at 24 to 72 hrs of incubation times. Conclusion: We concluded that Zinc shows cytotoxic effects (Raji & Molt-4) depending on the concentrations used. At higher concentrations, it exerts cytotoxic effects. SP - 32 EP - 37 AU - Tokmehdashi, H AU - Osati Ashtiani, F AU - Purfathullah, AA AD - KW - : B- Lymphaytes KW - T- Lymphocytes KW - Zinc UR - http://journal.gums.ac.ir/article-1-652-en.html ER -