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Research code: ۹۹۱۲۰۴۰۲
Ethics code: IR.GUMS.REC.1400.04
Ladan Hosseinnia1 
, Shabnam Pourmehdi1 , Zeinab Morsali1 , Mehdi Evasalipour2 , Saeed Ghasemi3 , Fatemeh Yousefbeyk *1
, Shabnam Pourmehdi1 , Zeinab Morsali1 , Mehdi Evasalipour2 , Saeed Ghasemi3 , Fatemeh Yousefbeyk *1
1- Department of Pharmacognosy, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran
2- Department of Pharmaceutical Biotechnology, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran
3- Department of Medicinal Chemistry, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran
2- Department of Pharmaceutical Biotechnology, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran
3- Department of Medicinal Chemistry, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran
Abstract: (620 Views)
Background: With a surge in antibiotic resistance, there is a growing effort to discover new antibiotic agents from natural resources. Considering that geographical conditions have a major impact on the biological activity of medicinal plants, investigating the antimicrobial activity of native species is a necessity.
Objective: This article aims to investigate the antibacterial and antifungal activities of six native species of Rostam Abad, Guilan province. Also, the impact of solvent polarity used for the extraction of bioactive compounds on antimicrobial activities was investigated.
Methods: The aerial parts of selected plant species including Cruciata laevipes, Galium aparine, Cirsium arvense, Scrophularia rostrate, Stachys annua, and S. byzantine were extracted with methanol using the percolation method. Then hexane, ethyl acetate and methanol fractions were prepared. The antibacterial and antifungal activities were tested using two methods: disc diffusion and broth microdilution.
Results: The methanol fraction of C. laevipes showed the best anti-E. coli activity (MIC 0.3 mg/ml). Total extracts of C. arvense and S. byzantine had the best activity against B. subtilis (MIC 0.3 mg/ml). The ethyl acetate fraction of C. laevipes showed the highest activity against both B. subtilis and S. aureus. Also, the ethyl acetate fraction of S. annua exhibited the strongest activity on B. subtilis. The only sample with antifungal activity was the ethyl acetate fraction of S. annua.
Conclusion: Among the samples tested, C. laevipes exhibited better antibacterial activity. Ethyl acetate proved to be more effective in extracting antimicrobial compounds, resulting in stronger activities. Additionally, further investigation is recommended to determine the bioactive phytochemicals and antibacterial mechanism of action.
Objective: This article aims to investigate the antibacterial and antifungal activities of six native species of Rostam Abad, Guilan province. Also, the impact of solvent polarity used for the extraction of bioactive compounds on antimicrobial activities was investigated.
Methods: The aerial parts of selected plant species including Cruciata laevipes, Galium aparine, Cirsium arvense, Scrophularia rostrate, Stachys annua, and S. byzantine were extracted with methanol using the percolation method. Then hexane, ethyl acetate and methanol fractions were prepared. The antibacterial and antifungal activities were tested using two methods: disc diffusion and broth microdilution.
Results: The methanol fraction of C. laevipes showed the best anti-E. coli activity (MIC 0.3 mg/ml). Total extracts of C. arvense and S. byzantine had the best activity against B. subtilis (MIC 0.3 mg/ml). The ethyl acetate fraction of C. laevipes showed the highest activity against both B. subtilis and S. aureus. Also, the ethyl acetate fraction of S. annua exhibited the strongest activity on B. subtilis. The only sample with antifungal activity was the ethyl acetate fraction of S. annua.
Conclusion: Among the samples tested, C. laevipes exhibited better antibacterial activity. Ethyl acetate proved to be more effective in extracting antimicrobial compounds, resulting in stronger activities. Additionally, further investigation is recommended to determine the bioactive phytochemicals and antibacterial mechanism of action.
Keywords: zone of inhibition, broth microdilution, Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Candida albicans, Aspergillus niger, Rostam Abad
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