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Research code: 92-6766
Ethics code: 92-6766
1- Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
2- Department of Microbiology, School of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran
2- Department of Microbiology, School of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran
Abstract: (244 Views)
Background Escherichia coli and Klebsiella spp. are amongst the most important causative agents of nosocomial and urinary tract infections. Integrons are genetic elements that carry genetic determinants for antimicrobial resistance and one of the important factors that can contribute to the increase of multi-drug resistant (MDR) bacteria.
objective The aim of the current study was to determine the frequency of integron-mediated antimicrobial resistance genes (intI) among E. coli and Klebsiella spp. isolated from clinical specimens in Shiraz Teaching hospitals.
Methods Two hundred isolates of E. coli and Klebsiella spp. were recovered from different clinical specimens between September 2012 and February 2013. Detection and confirmation of all of the isolates were performed by microbiological phenotypic tests and API 20E kit, respectively. The disk diffusion method was used to determine the susceptibility of 200 isolates against 12 antibiotics and class of integrons was detected in MDR isolates by PCR-RFLP assay.
Results Antibiogram showed the highest and the least resistance rate were toward to ampicillin (94.5%) and imipenem (6.5%), correspondingly. MDR were determined in 128 (64%) of isolates. Nineteen (14.8%) of MDR isolates were harbored intI gene and intII and intIII were not diagnosed in no isolates.
Conclusion This work revealed a high rate of antibiotic resistance among our isolates. Regarding the low frequency of the presence of int genes, it could be concluded that other genetic elements such as transposons are involved in resistant isolates.
objective The aim of the current study was to determine the frequency of integron-mediated antimicrobial resistance genes (intI) among E. coli and Klebsiella spp. isolated from clinical specimens in Shiraz Teaching hospitals.
Methods Two hundred isolates of E. coli and Klebsiella spp. were recovered from different clinical specimens between September 2012 and February 2013. Detection and confirmation of all of the isolates were performed by microbiological phenotypic tests and API 20E kit, respectively. The disk diffusion method was used to determine the susceptibility of 200 isolates against 12 antibiotics and class of integrons was detected in MDR isolates by PCR-RFLP assay.
Results Antibiogram showed the highest and the least resistance rate were toward to ampicillin (94.5%) and imipenem (6.5%), correspondingly. MDR were determined in 128 (64%) of isolates. Nineteen (14.8%) of MDR isolates were harbored intI gene and intII and intIII were not diagnosed in no isolates.
Conclusion This work revealed a high rate of antibiotic resistance among our isolates. Regarding the low frequency of the presence of int genes, it could be concluded that other genetic elements such as transposons are involved in resistant isolates.
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