Volume 16, Issue 64 (1-2008)                   JGUMS 2008, 16(64): 51-59 | Back to browse issues page

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Saraei Sahnesaraei M, Jahani Hashemi H. Toxoplasmosis Indirect Immunofluorescent Antibody TestAgreement Rate among Four Laboratory Centers. JGUMS 2008; 16 (64) :51-59
URL: http://journal.gums.ac.ir/article-1-344-en.html
1- , msaraei@qums.ac.ir
Abstract:   (6828 Views)
Abstract Introduction: Indirect immunofluorescent antibody (IFA) test is one of the most common serological tests for toxoplasmosis. Some of Toxoplasma seroepidemiological studies used this test in Iran. Review the studies which were performed by this test in different areas of the country with different climates, shows according to main way of transmission of Toxoplasma gondii in Iran, performing the test needed consideration. It seems that these probable contradictions are due to mistakes of the test procedure in some of the laboratories. Objective: This study was performed to determine the agreement rate of IFA toxoplasmosis test among 4 laboratory centers. Materials and Methods: The study is a multicenter evaluation. With the consideration of ethical values, serums were prepared from 100 subjects' who had reffered for pre-marriage examinations to Qazvin medical community center in the year 2006. Each serum divided to four parts and in each laboratory was examined for specific IgG anti-Toxoplasma antibodies with the IFA test. The tests in each center were performed according to common protocol of that center. The sera in all laboratories were examined at 1:20, 1:100, 1:200 and above. The titration ≥ 1:20 was considered as positive. The kappa coefficient between each 2 centers was calculated. Results: The seropositivity rates of IgG-IFA Toxoplasma test in the 4 centers were 72%, 44%, 34% and 23%. The kappa coefficient in comparing with 2 centers, for upper limit was 0.36 and for the lower limit was 0.16. Conclusion: In the present study, the agreement rates among the four centers were very lower than our expect. It seems that, performing this test in some of laboratories in country accompanied with some defects that should be corrected. Therefore, we recommend to survey separately.
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Review Paper: Research | Subject: Special
Received: 2013/12/24 | Accepted: 2013/12/24 | Published: 2013/12/24

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