Survey of Induced Apoptosis Effect of Ciprofloxacin in Rat&#039;s Testis by TUNEL Technique

Khaki, A; Ghafari novin , M; Ebrahim nezhad, A.A; Khaki , A.A

Volume 16, Issue 61 (4-2007) JGUMS 2007, 16(61): 22-29 | Back to browse issues page

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Khaki A, Ghafari novin M, Ebrahim nezhad A, Khaki A. Survey of Induced Apoptosis Effect of Ciprofloxacin in Rat's Testis by TUNEL Technique . JGUMS 2007; 16 (61) :22-29
URL: http://journal.gums.ac.ir/article-1-394-en.html

Survey of Induced Apoptosis Effect of Ciprofloxacin in Rat's Testis by TUNEL Technique

A Khaki ^*

¹, M Ghafari novin

, A.A Ebrahim nezhad

, A.A Khaki

1- , dr.khaki@iaut.ac.ir & arashkhaki@canada.com

Abstract: (7975 Views)

Abstract Introduction: Ciprofloxacin is a synthetic antibacterial agent belonging to the family of fluoroquinolones that has a very broad spectrum against of microbial pathogens, especially Gram-negative infectious diseases, which has been approved in more than 100 countries in world. Objective: The aim of this study was planed to see apoptosis effects of ciprofloxacin after inducement, in rat testis. Materials and Methods: The twenty male wistar rat were selected and divided randomly into two groups control (n=10) and experimental (n=10). The test group was received 12.5mg/kg (PO) ciprofloxacin daily for sixty days, in their drinking water however the control group just received its routine drinking water. On sixtieth day the testis tissue of rats in both groups were removed in 10% formalin solution and prepared for light microscopy, and the Terminal Uridine Nick End Labeling (TUNEL) technique was used to identify apoptosis. Results: Light microscopic studies showed apoptotic bodies in tissue of testis in experimental group were (15.15±10.17) and were(3±2.41) in control group, This difference in apoptotic bodies in experimental and control groups was significant (P<0.01). Conclusion: Since in our study ciprofloxacin had side effect such as increasing apoptotic body in testis cells in rat in experimental group, then it was suggested that ciprofloxacin using in human could induced many histopathogical disorders and might be decreased the fertility rat.

Keywords: Apoptosis, Ciprofloxacin, Rat, Testis

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Review Paper: Research | Subject: Special
Received: 2014/01/1 | Accepted: 2014/01/1 | Published: 2014/01/1

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