Abstract Introduction: The majority of carious lesions are not well-defined radiolucencies. Approximately 40% demineralization is required for radiographic detection of a lesion. The actual depth of penetration of carious lesion is deeper than may be detected radio graphically. However, digital subtraction images permit to detect 1-5% decrease of mineral mass per unit volume. Objective: The aim of this study was to detect central depth of dental demineralization on digital subtraction radiography. Materials and Methods: This study was preformed on 15 extracted human teeth. In each tooth, one approximal enamel demineralization defect was induced using an acidified system (PH=4.8). Direct digital radiography were obtained under standardized condition over a period of 42 days. The images of the 7th , 14th , 21st , 28th , 35th and 42nd days were subtracted from the baseline radiograph (before creation of the lesion). Because all of images showed moderate proximal demineralization on 42nd days, all teeth sectioned for histological study. Then, the mean standard deviation of the extent demineralization was obtained with the radiographic and histological measurements. The test served as the statistical sampling unit for testing difference between the measurements. Results: After 42 days, the mean±SD of the extent of demineralization was underestimated by radiographic assessment (0.48±0.25 mm than histometric measurement 0.54±0.18 mm).However this difference wasn't significant (P=0.82). Conclusion: For monitoring the progression of carries clinically, central depth seems to be the proper parameter.

Keywords: Dental Caries, Radiography, Dental, Tooth Demineralization

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