Introduction: GABA is a inhibitory neurotransmitter in CNS that plays an important role in the regulation of blood glucose. There have been reports that GABA-A receptors might cause a reduction in the concentration of blood glucose by increasing the insulin plasma and decreasing the glucagon and somatostatine. Objective: This study was carried out to compare the effects of interaperitoneal and intracerebellar ventricle injection of Picrotoxin and Biccuculine on regulation of blood glucose concentration in mice. Materials and methods: In this experimental study, male albino mice weighing 20-30 grams were used. Serial blood collection from each animal was done by retro- orbital sinus puncture, and glucose concentration was measured using O-toluidin. In this experiment, the effect of different doses (IP and ICV) injection of Picrotoxin and Biccuculine on blood glucose was studied. One-way variance analysis and t-test were adopted in statistical analysis of the collected data. Results: The results indicated that IP and ICV injection of Picrotoxin and Biccuculine induce significant increase of blood glucose concentration in two groups (p<0.05). Another point is that increased blood glucose concentration is observed until 45-th mintue in all doses but after this time, blood glucose almost according to similar pattern is reduced and in 90 minutes, it will reach its minimal level. Conclusion: Therefore, it seems that GABA- A receptors have an inhibiting role in regulation of blood glucose. Further research seems inevitable since the effects of GABA-A receptors on blood glucose concentration is a complex phenomenon.

Keywords: Bicucullin, Glucose, Picrotoxin

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