Volume 19, Issue 76 (1-2011)                   JGUMS 2011, 19(76): 29-37 | Back to browse issues page

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Farzin D, Mousavi E. Effects of Harmane, Norharmane and Harmine on Writhing Behavior Induced by Acetic Acid in Mice. JGUMS 2011; 19 (76) :29-37
URL: http://journal.gums.ac.ir/article-1-168-en.html
1- University of Medical Sciences , davoodfarzin@yahoo.com
2- University of Medical Sciences
Abstract:   (7380 Views)
Abstract Introduction: The b-carbolines harmane, harmine and norharmane are the members of Harmala,s alkaloids group (Peganum harmala, Zygophillaceae). The b-carboline alkaloids adjoined to benzodiazepine site of the γ-aminobutyric acid type A (GABAA). These alkaloids also inhibited cyclooxygenase and lipoxygenase activities. These findings showed that the b-carbolines should be able to reduce writhing nociceptio induced by acetic acid- in mice. Objective: To assess the effects of acute treatment with harmane, norharmane and harmine on the writhing induced by acetic acid in mice. Materials and Methods: The experiments were carried out on male BALB/C mice (20-25g). Intraperitoneal (I.p) injection of acetic acid (0.6%) was performed in order to cause writhing behavior. This behavior was recorded by direct observation for a 30-minutes period. Decrease of writhing count is indicative of an anti-nociception. In order to avoid the possibility of a physicochemical interaction between them, Drugs were administered on opposite sides of peritoned. Results: Intraperitoneal (I.p) injection of Harmane (5-20mg/kg) on 6-9 mice, norharmane (5-15mg/kg) on 8-9 mice and harmine (10-15mg/kg) on 8-9 mice in per group decreased the writhing behavior significantly (P<0.0001, P<0.0003 and P<0.0016, respectively). The inhibitory effects of the mentioned drugs were antagonized by flumazenil (2 mg/kg) Conclusion: Effects of harmane, norharmane and harmine on writhing response may be mediated through an inverse agonistic mechanism located in the benzodiazepine receptors.
Keywords: Pain, Carbolins, Harmine, Mouse
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Review Paper: Research | Subject: Special
Received: 2013/11/11 | Accepted: 2013/11/11 | Published: 2013/11/11

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