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Research code: 996
Ethics code: IR.GUMS.REC.1397.237
1- 1Cellular and Molecular Research Center, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran
Abstract: (315 Views)
Background:Hyaluronic acid (HA) is a natural glycosaminoglycan composed of N-acetylglucosamine and glucuronic acid, recognized as a key component of the extracellular matrix in various tissues. Studies have demonstrated that the molecular weight of HA significantly influences its biological functions.
Objective:This study aimed to investigate the effects of three molecular weights of HA—LMW-HA (less than 100 kDa), MMW-HA (100-500 kDa), and HMW-HA (more than 1000 kDa)—on the expression of inflammatory factors (IL-1β, TNF-α, IL-6) and CD86 in THP-1 cells.
Methods:THP-1 cells were treated with HA of different molecular weights and concentrations. Gene expression levels of IL-1β, TNF-α, and IL-6 were analyzed using Real-time PCR, protein levels were measured via ELISA, and CD86 expression was assessed through flow cytometry. Data were statistically analyzed using ANOVA and Dunnett's test.
Results:Treatment with LMW-HA and MMW-HA significantly increased the gene and protein expression of IL-1β, TNF-α, and IL-6 compared to the control group (P < 0.05). Conversely, HMW-HA at concentrations of 50 and 100 µg/mL significantly reduced the expression of these factors (P < 0.05). Moreover, LMW-HA and MMW-HA significantly enhanced CD86 expression, whereas no significant changes were observed with HMW-HA (P > 0.05).
Conclusion:The molecular weight of HA plays a critical role in modulating the expression of inflammatory factors and CD86, suggesting its potential application in targeted therapeutic strategies.
Objective:This study aimed to investigate the effects of three molecular weights of HA—LMW-HA (less than 100 kDa), MMW-HA (100-500 kDa), and HMW-HA (more than 1000 kDa)—on the expression of inflammatory factors (IL-1β, TNF-α, IL-6) and CD86 in THP-1 cells.
Methods:THP-1 cells were treated with HA of different molecular weights and concentrations. Gene expression levels of IL-1β, TNF-α, and IL-6 were analyzed using Real-time PCR, protein levels were measured via ELISA, and CD86 expression was assessed through flow cytometry. Data were statistically analyzed using ANOVA and Dunnett's test.
Results:Treatment with LMW-HA and MMW-HA significantly increased the gene and protein expression of IL-1β, TNF-α, and IL-6 compared to the control group (P < 0.05). Conversely, HMW-HA at concentrations of 50 and 100 µg/mL significantly reduced the expression of these factors (P < 0.05). Moreover, LMW-HA and MMW-HA significantly enhanced CD86 expression, whereas no significant changes were observed with HMW-HA (P > 0.05).
Conclusion:The molecular weight of HA plays a critical role in modulating the expression of inflammatory factors and CD86, suggesting its potential application in targeted therapeutic strategies.
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