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Research code: 95092014
Ethics code: IR.GUMS.REC.1395.304
1- Department of Pharmaceutical Sciences, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran
2- Department of Pharmacognosy, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran
3- Department of Medicinal Chemistry, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran,
2- Department of Pharmacognosy, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran
3- Department of Medicinal Chemistry, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran,
Abstract: (461 Views)
Background: The leaves of Cassia angustifolia Vahl. have been used for the treatment of constipation and bowel evacuation. The main pharmacologically active constituents are sennosides A (SA) and B (SB).
Objective: Six different laxative pharmaceutical preparations of C. angustifolia leaves from Iran were quantitatively analyzed by a UV-Visible spectrophotometric method. Also, a simple, rapid, and precise HPLC method was developed and validated for the determination SA and SB in these formulations.
Methods: The UV spectrophotometric quantification was performed by a method described in the British Pharmacopoeia. HPLC analysis was done using the Waters apparatus equipped with a photodiode array (PDA) detector. The reliability of the method was validated through assessments of its linearity, accuracy, inter-day and intra-day precision, limit of detection (LOD), and limit of quantitation (LOQ).
Results: The percentage of hydroxyanthracene derivatives expressed as SB ranged from 1.76 to 2.95% using UV-spectrophotometric method. The range of SA and SB was between 0.57% to 1.12% and 0.86% to 1.43%, respectively, as determined by the HPLC method. In the calibration curve, linearity was observed for both compounds between concentration range of 10-800 μg/mL. LODs and LOQs were calculated as 3.21 and 9.73, and 2.65 and 8.03 μg/mL for SA and SB, respectively. The precision results exhibited RSD% values of ≤ 3.84% and 3.81% for inter-day and ≤ 3.71% and 3.32% for intra-day for SA and SB, respectively.
Conclusion: The developed HPLC method is simple, rapid, precise, and cost-effective in terms of the time and the solvent usage (10 mL per analysis), and wide range of linearity.
Objective: Six different laxative pharmaceutical preparations of C. angustifolia leaves from Iran were quantitatively analyzed by a UV-Visible spectrophotometric method. Also, a simple, rapid, and precise HPLC method was developed and validated for the determination SA and SB in these formulations.
Methods: The UV spectrophotometric quantification was performed by a method described in the British Pharmacopoeia. HPLC analysis was done using the Waters apparatus equipped with a photodiode array (PDA) detector. The reliability of the method was validated through assessments of its linearity, accuracy, inter-day and intra-day precision, limit of detection (LOD), and limit of quantitation (LOQ).
Results: The percentage of hydroxyanthracene derivatives expressed as SB ranged from 1.76 to 2.95% using UV-spectrophotometric method. The range of SA and SB was between 0.57% to 1.12% and 0.86% to 1.43%, respectively, as determined by the HPLC method. In the calibration curve, linearity was observed for both compounds between concentration range of 10-800 μg/mL. LODs and LOQs were calculated as 3.21 and 9.73, and 2.65 and 8.03 μg/mL for SA and SB, respectively. The precision results exhibited RSD% values of ≤ 3.84% and 3.81% for inter-day and ≤ 3.71% and 3.32% for intra-day for SA and SB, respectively.
Conclusion: The developed HPLC method is simple, rapid, precise, and cost-effective in terms of the time and the solvent usage (10 mL per analysis), and wide range of linearity.
Keywords: Cassia angustifolia, sennoside A, sennoside B, HPLC analysis, UV-Visible spectrophotometry
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