Volume 34, Issue 4 (12-2025)
JGUMS 2025, 34(4): 0-0 |
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Research code: 0
Ethics code: IR.YAZD.REC.1402.058
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Zamani F. The effect of Tumstatin and Canstatin-derived angiogenesis inhibitor peptides on FAK expression in animal model of breast cancer. JGUMS 2025; 34 (4)
URL: http://journal.gums.ac.ir/article-1-2776-en.html
URL: http://journal.gums.ac.ir/article-1-2776-en.html
1- Department of Biology, Yazd University, Yazd, Iran
Abstract: (412 Views)
Background: Canstatin and Tumstatin are proteins derived from Collagen IV that inhibit tumor growth by preventing angiogenesis. Increased Focal Adhesion Kinase (FAK) expression may promote tumor progression and metastasis by stimulating cancer cell proliferation and angiogenesis.
Objectives: This study investigates the effects of peptides derived from Canstatin and Tumstatin on breast tumor growth in an animal model and on the expression of FAK in tumor tissue.
Methods: Tumors were induced in mice by injecting 4T1 cell lines into their flanks. The treatment groups were injected intraperitoneally 5 mg/kg/day of the peptides for 12 days, while the control group was given an equal volume of buffer. Tumor sizes were measured, and tumor volume was calculated using the v=(the smallest diameter)2×(the largest diameter)×0.52 formula. In the end, the tumors were removed, and RNA was extracted from the tissues. cDNA synthesis was performed using primers designed for GAPDH as a reference gene and FAK and PCR methods. The gene expression was measured using RT-PCR and ΔCT calculation for treatment and control samples.
Results: The peptides derived from Canstatin and Tumstatin inhibited tumor growth by 51% and 49%, respectively, compared to the control group (P<0.05). Additionally, these peptides reduced FAK expression by 69% and 67%, respectively, compared to the control (P <0.05).
Conclusion: The present study confirmed the antitumor effect of these peptides and demonstrated that FAK expression reduction in tumors may be one of their antiangiogenic and anticancer mechanisms. The present results may help to design new anticancer peptide drugs.
Objectives: This study investigates the effects of peptides derived from Canstatin and Tumstatin on breast tumor growth in an animal model and on the expression of FAK in tumor tissue.
Methods: Tumors were induced in mice by injecting 4T1 cell lines into their flanks. The treatment groups were injected intraperitoneally 5 mg/kg/day of the peptides for 12 days, while the control group was given an equal volume of buffer. Tumor sizes were measured, and tumor volume was calculated using the v=(the smallest diameter)2×(the largest diameter)×0.52 formula. In the end, the tumors were removed, and RNA was extracted from the tissues. cDNA synthesis was performed using primers designed for GAPDH as a reference gene and FAK and PCR methods. The gene expression was measured using RT-PCR and ΔCT calculation for treatment and control samples.
Results: The peptides derived from Canstatin and Tumstatin inhibited tumor growth by 51% and 49%, respectively, compared to the control group (P<0.05). Additionally, these peptides reduced FAK expression by 69% and 67%, respectively, compared to the control (P <0.05).
Conclusion: The present study confirmed the antitumor effect of these peptides and demonstrated that FAK expression reduction in tumors may be one of their antiangiogenic and anticancer mechanisms. The present results may help to design new anticancer peptide drugs.
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Received: 2025/03/1 | Accepted: 2025/05/5 | Published: 2025/12/31
Received: 2025/03/1 | Accepted: 2025/05/5 | Published: 2025/12/31
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