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Research code: 27763
Ethics code: IR.SUMS.MED.REC.1402.150
Sajad Vatany Setoodeh1 
, Fatemeh Askari1 , Keivan Sahebi2 , Seyed Mahmoud Sadjjadi1 , Yosef Sharifi1 , Rasoul Alimi3 , Aref Teimouri *4
, Fatemeh Askari1 , Keivan Sahebi2 , Seyed Mahmoud Sadjjadi1 , Yosef Sharifi1 , Rasoul Alimi3 , Aref Teimouri *4
1- Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
2- Social Determinants of Health Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
3- Department of Epidemiology and Biostatistics, School of Health, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
4- Department of Parasitology and Mycology, Basic Sciences in Infectious Diseases Research Center, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
2- Social Determinants of Health Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
3- Department of Epidemiology and Biostatistics, School of Health, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
4- Department of Parasitology and Mycology, Basic Sciences in Infectious Diseases Research Center, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Abstract: (304 Views)
Background: Cystic echinococcosis is a parasitic zoonotic disease, prevalent in many parts of the world. The pharmacological treatment of this disease is often challenged by the adverse effects of available drugs.
Objective: This study aimed to evaluate the in vitro effect of the hydroalcoholic extract of licorice (Glycyrrhiza glabra L.) on hydatid cyst protoscoleces.
Methods: The hydroalcoholic extract of G. glabra was obtained from the dried roots of licorice using the percolation method. The extract was prepared at concentrations of 5, 10, 20, 40, and 80 mg/mL and exposed to protoscoleces for 10, 20, 30, 60, and 120 minutes. The mortality rate of the protoscoleces was assessed using eosin staining and light microscopy. Phosphate-buffered saline (PBS) and hypertonic saline (20%) solution were used as negative and positive controls, respectively.
Results: After 120 minutes, the hydroalcoholic extract of licorice killed 18.67 ± 1.53% of protoscoleces at a concentration of 5 mg/mL. The highest protoscolicidal effect (75.00 ± 3%) was observed at 80 mg/mL after 120 minutes, both showing statistically significant differences compared to the negative control group (p < 0.001).
Conclusion: The findings suggest that the hydroalcoholic extract of licorice could be potential considered as a promising protoscolicidal agent. However, further research, particularly in in vivo and clinical models, is necessary to confirm its efficacy and evaluate its side effects and safety profile.
Objective: This study aimed to evaluate the in vitro effect of the hydroalcoholic extract of licorice (Glycyrrhiza glabra L.) on hydatid cyst protoscoleces.
Methods: The hydroalcoholic extract of G. glabra was obtained from the dried roots of licorice using the percolation method. The extract was prepared at concentrations of 5, 10, 20, 40, and 80 mg/mL and exposed to protoscoleces for 10, 20, 30, 60, and 120 minutes. The mortality rate of the protoscoleces was assessed using eosin staining and light microscopy. Phosphate-buffered saline (PBS) and hypertonic saline (20%) solution were used as negative and positive controls, respectively.
Results: After 120 minutes, the hydroalcoholic extract of licorice killed 18.67 ± 1.53% of protoscoleces at a concentration of 5 mg/mL. The highest protoscolicidal effect (75.00 ± 3%) was observed at 80 mg/mL after 120 minutes, both showing statistically significant differences compared to the negative control group (p < 0.001).
Conclusion: The findings suggest that the hydroalcoholic extract of licorice could be potential considered as a promising protoscolicidal agent. However, further research, particularly in in vivo and clinical models, is necessary to confirm its efficacy and evaluate its side effects and safety profile.
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