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Research code: 3201
Ethics code: 32
Marzyeh Ghayoumian1 
, Adeleh Alihashemi2 , Azadeh Kabiri1 , Maryam Omidioskuie1 , Iraj Nikokar1 , Faezeh Adibian1 , Korosh Khanaki *3
, Adeleh Alihashemi2 , Azadeh Kabiri1 , Maryam Omidioskuie1 , Iraj Nikokar1 , Faezeh Adibian1 , Korosh Khanaki *3
1- Medical Biotechnology Research Center, School of Nursing, Midwifery and Paramedicine, Guilan University of Medical Sciences, Rasht, Iran
2- Department of biology and biotechnology “Lazzaro spallanzi”, university of Pavia/ unipv, Pavia, Italy
3- Department of Clinical Biochemistry, Guilan University of Medical Sciences, Rasht, Iran
2- Department of biology and biotechnology “Lazzaro spallanzi”, university of Pavia/ unipv, Pavia, Italy
3- Department of Clinical Biochemistry, Guilan University of Medical Sciences, Rasht, Iran
Abstract: (124 Views)
Background: Breast cancer accounts for about 11.6 % of all cancer cases. The high cost and adverse effects of conventional chemotherapy have led to growing interest in low-cost, plant-based therapeutics. Sporamin, a protease inhibitor found in sweet potatoes (Ipomoea batatas), has shown potential as an anticancer agent.
Objective: This study aimed to evaluate the pro-apoptotic effects of purified sporamin and its impact on the expression of apoptosis-related genes (Fas, Bax, p53, and Bcl-2) in MCF-7 breast cancer cells.
Methods: MCF-7 cells were treated with 250 and 950 µg/ml of purified sporamin for 24 and 48 hours, respectively. Apoptosis was assessed using Annexin V/PI flow cytometry, and gene expression changes were analyzed by quantitative real-time PCR (qRT-PCR).
Results: After 24 and 48 hours of treatment, early apoptosis was observed in ~31% and ~37% of cells, and late apoptosis in 10% and 16%, respectively (P < 0.01). Sporamin significantly upregulated Fas, Bax, and p53 (5.6-, 2.15-, and 3.63-fold at 24h; 3.4-, 2.7-, and 6.42-fold at 48h), while downregulating Bcl-2 (4.42-fold at 24h and 2.92-fold at 48h) (P < 0.05).
Conclusion: Sporamin induces apoptosis in MCF-7 cells through both intrinsic and extrinsic pathways by modulating key regulatory genes. These results highlight sporamin’s potential as a cost-effective, plant-derived candidate for further preclinical evaluation in breast cancer therapy.
Objective: This study aimed to evaluate the pro-apoptotic effects of purified sporamin and its impact on the expression of apoptosis-related genes (Fas, Bax, p53, and Bcl-2) in MCF-7 breast cancer cells.
Methods: MCF-7 cells were treated with 250 and 950 µg/ml of purified sporamin for 24 and 48 hours, respectively. Apoptosis was assessed using Annexin V/PI flow cytometry, and gene expression changes were analyzed by quantitative real-time PCR (qRT-PCR).
Results: After 24 and 48 hours of treatment, early apoptosis was observed in ~31% and ~37% of cells, and late apoptosis in 10% and 16%, respectively (P < 0.01). Sporamin significantly upregulated Fas, Bax, and p53 (5.6-, 2.15-, and 3.63-fold at 24h; 3.4-, 2.7-, and 6.42-fold at 48h), while downregulating Bcl-2 (4.42-fold at 24h and 2.92-fold at 48h) (P < 0.05).
Conclusion: Sporamin induces apoptosis in MCF-7 cells through both intrinsic and extrinsic pathways by modulating key regulatory genes. These results highlight sporamin’s potential as a cost-effective, plant-derived candidate for further preclinical evaluation in breast cancer therapy.
Keywords: Sporamin, Ipomoea batatas, Apoptosis, Serine Protease Inhibitors, MCF-7 Cells, Gene Expression Regulation
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